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2. S E Y M O U R Polysaccharide Branching 37 I f the hypothesis s t a t i n g that the resonances o f each r e s i d u e type were independent o f adjacent r e s i d u e s t r u c t u r e s , then the C - l resonance o f the terminal r e s i d u e would be easy to i d e n t i f y by comparing the a d d i t i o n a l branching anomeric resonances o f 2-branching, 3-branching, and 4-branching, thereby i d e n t i f y i n g the c o n s i s t e n t minor resonance. T h i s i s not the case, as a comparison o f the s e r i e s o f dextrans o f d i f f e r e n t branching type demonstrates.

Furthermore, the t o t a l spectrum o f the s y n t h e t i c a l l y branched amyloses was e s s e n t i a l l y a composite o f the amylose resonances p l u s the methyl 3-D-glucopyranoside resonances. Two p o i n t s were o f i n t e r e s t i n t h i s study. m. m. m. (the 4 , 6 - d i - 0 - s u b s t i t u t e d α-D-glucopyranosyl r e s i d u e ) . As was p r e v i o u s l y shown f o r a-(1+3)-linkages f o r dextran, t h i s again i n d i c a t e s that the d i a g n o s t i c resonances are dependent on the r e s i d u e type present, and not on the linkage type.

Due t o the corresponding terminal α-D-gluco­ pyranosyl residue a s s o c i a t e d with each branch-point r e s i d u e , i t could be expected that each branching type could c o n t r i b u t e twelve (2x6) minor resonances t o the dextran s p e c t r a , and that the "minor" resonances would s t e a d i l y become more intense (compared t o the l i n e a r dextran resonances) as the polymer becomes more h i g h l y branched. m. r e g i o n . m. r e g i o n presents a second complementary s p e c t r a l r e g i o n f o r s t r u c t u r a l a n a l y s i s .

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