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By Farrington C.P., Kanaan M.N.

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4. Discard the acetonitrile and vacuum centrifuge for 3–5 min (see Note 18). 5. Add 50 µL of reducing solution at room temperature for 30 min. 6. Remove the reducing solution and add 50 µL of alkylation solution at room temperature for 30 min. 7. Remove the alkylation reagent and dehydrate the gel pieces in 200 µL of acetonitrile. 8. Remove the acetonitrile. The gel pieces should shrink and become an opaque-white color. If they do not, remove the acetonitrile and repeat the washing–dehydration cycle until they do.

This probability serves as an initial (pretest) probability that directs what diagnostic test(s) and/or procedures are to be ordered, and then the results from the tests and procedures are used to modify this probability until the post-test probability is such that either the suspected diagnosis is confirmed or ruled out. Therefore, the value of a diagnostic test is often assessed in terms of how accurate this updating of information reveals the true disease status of the patient. Sox et al. (30) pointed out that the main purposes of a diagnostic test are: (1) to provide reliable information about the patient’s condition and (2) to influence the health care provider’s plan for managing the patient.

36. Tan, P. , Downey, T. , E. , et al. (2003) Evaluation of gene expression measurements from commercial microarray platforms. Nucleic Acids Res. 31, 5676–5684. 37. , et al. (2004) A comparison of cDNA, oligonucleotide, and Affymetrix GeneChip gene expression microarray platforms. J. Biomol. Tech. 15, 276–284. 38. , Beyer, R. , et al. (2005) Standardizing global gene expression analysis between laboratories and across platforms. Nat. Methods 2, 351–356. 39. Larkin, J. , Frank, B. , and Quackenbush, J.

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Branching process models for surveillance of infectious diseases controlled by mass vaccination by Farrington C.P., Kanaan M.N.

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