By Ivan Krhen M.D., Ph.D., Andreja Vukasović M.D. (auth.), Davor Ježek (eds.)
Atlas at the Human Testis: basic Morphology and Pathology provides histological illustrative fabric from paraffin and semi-thin sections of the human testis that are mostly utilized in the evaluate of testicular morphology, permitting an early detection of carcinoma in situ and extra complex pathological adjustments of the testicular parenchyma. The early detection of melanoma in situ relies at the cautious morphological research of the biopsy and immunohistochemistry (IHC). for this reason, this atlas comprises distinct descriptions of IHC tools in addition to glossy molecular organic tools similar to DNA microarrays and proteomics and complex microscopy innovations relating to the testicular biopsy.
Adequate assessment of the testicular biopsy ends up in excessive treatment premiums of testicular neoplasms which are used as a foundation to effectively deal with infertility in males.
Atlas at the Human Testis: basic Morphology and Pathology is a invaluable reference instrument to be able to entice andrologists, urologists, pathologists, scientific embryologists, in addition to reproductive biology scientists.
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Extra resources for Atlas on the Human Testis: Normal Morphology and Pathology
DA dorsal aorta, H hindgut, M mesenterium, N notochord, PCV posterior cardinal vein, SC spinal cord, SG spinal ganglion (hematoxylin and eosin [H&E] stain; scale bar, 100 mm) 4 Early Development of the Human Testis Fig. 2 Transverse section from a 5-week-old human embryo. The urogenital ridge is a transitory structure formed by the gonadal ridge (GR), mesonephros (MSN), and mesoand paramesonephric ducts. Branches of the aorta (DA) form the mesonephric glomeruli (MG), which drain into the 39 posterior cardinal vein (PCV).
Langman’s medical embryology. 11th ed. Philadelphia: Lippincott William and Wilkins; 2010. p. 235–63. 4. Duančić V. Development of the urogenital system. In: Švajger A, editor. Basic human embryology [in Croatian]. 9th ed. Zagreb: Medicinska knjiga BeogradZagreb; 1985. p. 129–47. 5 The Fetal Human Testis Marina Kos and Tanja Leniček At our pathology department, all spontaneously aborted fetuses are routinely autopsied and analyzed. During fetal autopsy, the position of the testes is assessed and noted, and both testes are taken for routine histopathologic analysis.
11 For antigen retrieval, citrate buffer is usually applied Fig. 13 A primary antibody (PA) is applied to the sections. The primary antibody should be added carefully to cover the whole section (arrow) 3 Histologic Procedures and Testicular Biopsy Freezing Fig. 14 Immunohistochemical staining. The IHC procedure should be adjusted carefully according to different primary and secondary antibodies or kits. The appropriate 29 positive and negative controls/slides should be used for each IHC staining 30 A.
Atlas on the Human Testis: Normal Morphology and Pathology by Ivan Krhen M.D., Ph.D., Andreja Vukasović M.D. (auth.), Davor Ježek (eds.)